I assume you mean E2 (E in the second octave), that the surrounding notes D2, Eb2, and F2 are OK. 1. Have a qualified teacher evaluate your playing habits. 2. Try a different mouthpiece to see if the design of yours is accentuating the problem. 3. Some players use tuning crescents in the E tone hole. (see www.musicmedic.com for a description of cork crescents or email info@martinmods for info on brass, body contour tuning crescents) 4. A bore insert (a blob of hot glue at the right spot, probably just above the E palm key tone hole will lower the E2 and not affect E1.) 5. See someone who knows how to analyze and align the resonances of the instrument. A sharp B2 and/or E3 harmonic can make the E2 (overblown E1) sharp. #3 and #4 may improve the situation but may only be only compensating for the combined effects of other problems, while adding more little problems of their own. In person is best. Email [email protected] to set up a free Skype session.